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Spring 2020
Mar 29, 2024
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Information Select the Course Number to get further detail on the course. Select the desired Schedule Type to find available classes for the course.

CHEM 0313 - Biochemistry Laboratory
*Biochemistry Laboratory* Experimental biochemistry emphasizing the isolation, purification and characterization of enzymes and the cloning of genes and expression of recombinant protein. Traditional biochemical techniques such as UV-VIS spectroscopy, gel filtration, ion exchange and affinity chromatography, electrophoresis, and immunoblotting will be used in the investigation of several enzymes. Specific experiments will emphasize enzyme purification, enzyme kinetics, and enzyme characterization by biochemical and immunochemical methods. Major techniques in molecular biology will be introduced through an extended experiment that will include DNA purification, polymerase chain reaction, bacterial transformation, DNA sequencing, and the expression, purification, and characterization of the recombinant protein. Class discussions emphasize the underlying principles of the biochemical and molecular techniques employed in the course, and how these experimental tools are improved for particular applications. Laboratory reports stress experimental design, data presentation, and interpretation of results. (CHEM 0322) 2 hr. lect., 6 hrs. lab.
0.000 OR 1.000 Credit hours
0.000 OR 1.000 Lecture hours
0.000 TO 2.000 Other hours

Levels: Undergraduate
Schedule Types: Lab, Lecture
All Sections for this Course

Natural Sciences Division
Chemistry & Biochemistry Department

Course Attributes:
CW


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